古紙混合汚泥コンポスト中の植物生長促成作用に関わる糸状菌とそれらの代謝産物
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[Synopsis] Sometime, the compost products made from bio-sludge and containing paper strips, showed plant growth promoting effect by field application. We have evaluated to separating various fungi from biosludge compost that have PGPR (Plant Growth Promoting Rhizobacteria) effect against some crops by inoculating test into the incubation soil. Test fungi separated from the compost using multiple dilution method and colonization on the PDA agar plate. And, the surface of autoclaved oat serials kept each fungus and inoculated by mixing 3％ w/w to incubation soil for PGPF (Plant Growth Promoting Fungi) evaluation. As a result, we have recognized PGPF effect and have identified by 18S-rDNA analysis, following similarity analysis compared with on-line database. All tested fungi showed PGPF effect against cucumber plant compared from growth of the control plant. The compost made from garbage and old office paper strips by experimental compost machine sat on the faculty of agriculture, Kinki University, Nara city, The most PGPF effective species was identified as Gibberella sp. with 18S-rDNA analyses. And the antibacterial compounds produced by this fungus, was separated as red crystal. This compound identified as javanicin, one of Fusarium pigment by NMR and MS spectrum analyses. The Gibberella sp. did not inhibit the plant growth ofJ1 susceptible tomato cultivar. These data suggested that various fungi maybe effect on the plants growth promoter in the field. Though, some metabolites produced by these fungi showed antibacterial activity, these metabolites maybe act as suppressor of the soil borne plant disease.
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