汚泥コンポストの植物生長促成活性に関わる微生物と、それらの代謝産物

概要

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[Synopsis] Sometime, the compost products made from bio-sludge and containing paper strips, showed plant growth promoting effect in the field. We have evaluated to separating various fungi from biosludge compost that have PGPR (Plant Growth Promoting Rhizobacteria) effect against some cropsinoculating test into the incubation soil. Test fungi separated from the compost using by multiple dilution method and colonization on the PDA agar plate. And, the surface of autoclaved oat serials kept each fungus and inoculated by mixing 3％ w/w to incubation soil for PGPF (Plant Growth Promoting Fungi) evaluation. As a result, we have recognized PGPF effect and have identified by 18S-rDNA analysis, following similarity analysis compared with on-line database. All tested fungi showed PGPF effect against cucumber plant compared from growth of the control plant. And the fungus have produced red pigment, have identified as Penicillium sp. by 18S-rDNA analysis. The red pigment have isolated and identified as naphthoquinone dimmer, xanthomegnin using by EtOAc extraction of mycelium and silica gel column chromatography separation. These data suggested that various fungi maybe effect on the plants growth in the field. Though, some metabolites produced by these fungi showed antibacterial activity, these metabolites maybe act as suppressor of the soil borne plant disease.