Origin and function of the stalk-cell vacuole in Dictyostelium.
スポンサーリンク
概要
- 論文の詳細を見る
Large vacuoles are characteristic of plant and fungal cells, and their origin has long attracted interest. The cellular slime mould provides a unique opportunity to study the de novo formation of vacuoles because, in its life cycle, a subset of the highly motile animal-like cells (prestalk cells) rapidly develops a single large vacuole and cellulosic cell wall to become plant-like cells (stalk cells). Here we describe the origin and process of vacuole formation using live-imaging of Dictyostelium cells expressing GFP-tagged ammonium transporter A (AmtA-GFP), which was found to reside on the membrane of stalk-cell vacuoles. We show that stalk-cell vacuoles originate from acidic vesicles and autophagosomes, which fuse to form autolysosomes. Their repeated fusion and expansion accompanied by concomitant cell wall formation enable the stalk cells to rapidly develop turgor pressure necessary to make the rigid stalk to hold the spores aloft. Contractile vacuoles, which are rich in H(+)-ATPase as in plant vacuoles, remained separate from these vacuoles. We further argue that AmtA may play an important role in the control of stalk-cell differentiation by modulating the pH of autolysosomes.
- 2011-04-01
論文 | ランダム
- 技術進歩の下での最適取替え間隔に関する研究 : コスト面からの分析
- Usefulness of microvolt T wave alternans as a predictor of ventricular tachyarrhythmias : A prospective study using atrial pacing
- Ventricular Arrhythmias With Left Bundle Branch Block Pattern and Inferior Axis : Assessment of Their Mechanisms on the Basis of Response to ATP, Nicorandil and Verapamil
- 57 高温熱風炉の建設について(熱風炉・レンガ・高炉操業・その他, 製銑, 日本鉄鋼協会第 72 回(秋季)講演大会講演論文集 (I))
- 55 高温熱風炉の昇温および操業経過について(熱風炉・レンガ・高炉操業・その他, 製銑, 日本鉄鋼協会第 72 回(秋季)講演大会講演論文集 (I))