Influence of preparation method on polynucleotide conformation and pharmacological activity of lipoplex
スポンサーリンク
概要
- 論文の詳細を見る
Conformations of polyinosinic acid [poly(I)] and polycytidylic acid [poly(C)] in liposomes (lipoplex) were investigated by both circular dichroism (CD) spectroscopy and fluorescence resonance energy transfer (FRET) measurements, and compared with those in aqueous solution. The results indicate that poly(I) and poly(C) take double-stranded structure in aqueous solution at pH 6.5-7.5 in the presence of NaCl at higher concentration than 50 mM. Although lipoplex was prepared without NaCl to avoid aggregation of lipoplex particles, poly(I) and poly(C) were double-stranded in pre-mixed poly(I)/poly(C) lipoplex (pre-mixed LIC), prepared by adding a mixed solution of poly(I) and poly(C) to the cationic liposomes. However, poly(I) and poly(C) did not take double-stranded structure in separately mixed LIC, prepared by separate addition of poly(I) solution and poly(C) solution to the cationic liposomes. The physicochemical properties (particle diameter and zeta potential) of pre-mixed LIC and separately mixed LIC were not different, but the anti-proliferative effect of pre-mixed LIC on human epidermoid carcinoma A431 cells was about eight times greater than that of separately mixed LIC. Our results indicate that polynucleotide conformation in lipoplex is markedly influenced by the preparation method, and the polynucleotide conformation in lipoplex has a substantial effect on pharmacological activity. © 2010 Elsevier B.V. All rights reserved.
- 2011-03-15
論文 | ランダム
- S3-1 転写因子NFATを標的としたアレルギー疾患治療(アレルギーにおける新規エフェクター分子標的薬の可能性を探る,第59回日本アレルギー学会秋季学術大会)
- 外国大学通信 アメリカの大学は変わったか(2)教育の質と研究条件
- 外国大学通信 アメリカの大学は変わったか(1)高等教育へのアクセス
- ウイリアム・デラムの『自然神学』
- 不整脈とイオンチャネル病 (第1土曜特集 ここまで進んだ 不整脈研究の最新動向) -- (不整脈研究の最先端)