Immunohistochemical observations of vitellin synthesis and accumulation processes in ovary of Ezo abalone Haliotis discus hannai
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Ovarian follicle cells are the site of yolk protein synthesis in the Ezo abalone Haliotis discus hannai. In this study, histological observations of ovarian follicle cells were conducted in H. discus hannai by immunocytochemical and in situ hybridization methods focusing on their function of yolk protein synthesis. An antibody raised against purified yolk protein (vitellin, Vn) recognized yolk granules in oocytes, and ovarian follicle cells adjacent to the oocytes under yolk accumulation were stained positively with both anti-Vn antibody and an antisense probe for vitellogenin (the precursor for vitellin, Vtg) mRNA. These results indicate that the abalone Vtg gene is transcribed and translated in the ovarian follicle cells. In oocytes in the early phase of yolk accumulation, positive reactions with the antibody appeared first in the stalk part, and the follicle cells adjacent to the stalk were also stained positively. These observations imply local transportation of yolk protein from the follicle cells to the oocyte through an extracellular space around the oocyte stalk. Ovarian follicle cells changed their morphological characteristics and reactivity to the antibody with close relationships with the stages of the adjacent oocyte, which suggests the presence of functional interactions between follicle cells and oocytes in the course of yolk protein synthesis and accumulation.エゾアワビ卵巣濾胞細胞（濾胞細胞）の卵黄タンパク質（ビテリン、Vn）合成と卵母細胞へのVn蓄積過程について、抗Vn抗体による免疫染色とVn前駆体遺伝子のin situ ハイブリダイゼーションで組織学的に検討した。その結果、前駆体遺伝子が濾胞細胞で転写、翻訳されることが示され、合成された卵黄タンパク質は濾胞細胞と卵母細胞が接する卵柄部で卵母細胞内に輸送されると示唆された。また濾胞細胞の形態と抗Vn抗体への反応性は隣接する卵母細胞の発達と密接に関連し、Vn合成と蓄積において濾胞細胞と卵母細胞間に相互作用が存在すると推定された。
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