光合成活性に応じた転写調節に関わる転写制御因子の解析 : 質量分析装置Autoflex IIIを用いて《マイレビュー》

概要

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The redox state of the photosynthetic electron transport chain acts as a critical sensing mechanism by regulating the transcription of key genes involved in the acclimation response to a change in the environment. We have shown that small LuxR-type regulator, PedR, is involved in photosynthetic electron transport-dependent transcriptional regulation in the cyanobacterium Synechocystis sp. PCC 6803. We report here that thioredoxin (Trx) is identified as an interacting factor of PedR by pull-down assays and subsequent MALDI-TOF MS spectrometry analysis using Autoflex III. Under high-light conditions, it is likely that PedR is transiently inactivated upon reduction by reducing equivalents generated at the acceptor side of photosystem I and mediated by Trx. This is the first report showing the direct interaction between Trx and a transcriptional regulator to connect the redox state of the photosynthetic electron transport chain with changes in gene expression.