A novel strictly NADPH-dependent Pichia stipitis xylose reductase constructed by site-directed mutagenesis.
スポンサーリンク
概要
- 論文の詳細を見る
Xylose reductase (XR) and xylitol dehydrogenase (XDH) are the key enzymes for xylose fermentation and have been widely used for construction of a recombinant xylose fermenting yeast. The effective recycling of cofactors between XR and XDH has been thought to be important to achieve effective xylose fermentation. Efforts to alter the coenzyme specificity of XR and HDX by site-directed mutagenesis have been widely made for improvement of efficiency of xylose fermentation. We previously succeeded by protein engineering to improve ethanol production by reversing XDH dependency from NAD(+) to NADP(+). In this study, we applied protein engineering to construct a novel strictly NADPH-dependent XR from Pichia stipitis by site-directed mutagenesis, in order to recycle NADPH between XR and XDH effectively. One double mutant, E223A/S271A showing strict NADPH dependency with 106% activity of wild-type was generated. A second double mutant, E223D/S271A, showed a 1.27-fold increased activity compared to the wild-type XR with NADPH and almost negligible activity with NADH.
- 2011-01-14
論文 | ランダム
- はんだ付技術の現状と動向--電子工業におけるはんだ付技術の進歩と問題点
- 最近のろう接技術
- MR-Mirror : 鏡像を用いた仮想世界と現実世界の融合提示(MR/ARの実用化に向けたCV/PR技術の課題と展望)
- 複数特徴部分空間法による手書き類似漢字識別
- 重複領域を有する複数の部分画像からの原画像再構成