Ehrlich腹水癌細胞の薬剤耐性細胞における温熱によるAdriamycinの効果増強とその修飾
スポンサーリンク
概要
- 論文の詳細を見る
Adriamycin (ADR) is a well known anticancer agent which is frequently used alone or combination with other anticancer agents. An ADR-resistant cell line derived from Ehrlich ascites tumor cells (EATC) was established in our laboratory. In this report, differences between wild EATC and ADR resistant EATC were studied. The intracellular ADR uptake increased at a temperature (41.0°C) that had a little effect on the viability of wild EATC. The intracellular ADR uptake of ADR-resistant EATC also increased at elevated temperature (41.0°C). However, ADR resistant EATC had an enhanced acquired capacity that increased the efflux of intracellular ADR. Cepharanthine (CP), 1mcg/ml, inhibited the efflux of intracellular ADR and maintained the retention of ADR at high levels in cells from both cell lines, so the killing effects were enhanced with a combination ADR and CP. From these results, the mechanisms of resistance to ADR may be discussed. The killing effects of ADR depend on intracellular ADR uptake, retention and maintenance. Hyperthermic treatment accelerates the influx of ADR. In addition, CP suppresses the efflux of intracellular ADR, and increased markedly the cytotoxic effect of ADR on ADR resistant EATC. The combination of ADR, CP and hyperthermia may be effective and useful therapy to overcome ADR-resistant cancer cells.
論文 | ランダム
- 1C1415 都市大気における NO-NO_2 光化学定常状態
- マルチモーメント・スキームCIP/IDO法を用いた大規模高精度流体計算 (特集 放電学会春季シンポジウム プラズマシミュレーションの最前線--放電はどこまでシミュレーションできるか?)
- 2 CIP法によるレーザーアブレーション過程のシミュレーション(シンポジウムVI : レーザーアブレーションプラズマの基礎と応用)
- E123 X 線レーザー媒体プラズマ生成過程シミュレーション
- CIP法を用いたプラズマのAnimationシミュレーション : CD-ROM特別企画 動画を用いたプラズマダイナミックスの研究