d-Fructose detection based on the direct heterogeneous electron transfer reaction of fructose dehydrogenase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode

概要

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Multi-walled carbon nanotubes (MWCNTs) were synthesized on platinum plate electrodes bythe chemical vapor deposition (CVD) method. From the results of X-ray photoelectronspectroscopy and voltammetric investigation, the iron nanoparticles used as a catalyst for theMWCNT synthesis were enclosed with MWCNTs. The MWCNTs synthesized on the Pt plate(MWCNTs/Pt) electrode were immediately immersed into solutions of D-fructose dehydrogenase(FDH) to immobilize the enzyme onto the MWCNTs/Pt electrode surfaces. After the FDH wasimmobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current based onFDH was observed from ca. -0.15 V (vs. Ag/AgCl/sat’d KCl), which was close to the redoxpotential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic currentvs. substrate, the calibration range for the fructose concentration was up to ca. 40 mmol dm-3, andthe apparent Michaelis-Menten constant was evaluated to be 11 (1) mmol dm-3.
2009-01-01