In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers.
スポンサーリンク
概要
- 論文の詳細を見る
Hepatocytes derived from human embryonic stem cells (hESCs) are an attractive cell source for regenerative medicine. We previously reported the differentiation of hESCs into alpha-fetoprotein (AFP)-producing endodermal cells by using extracellular matrix and growth factors. We also reported the establishment of the MLSgt20 cell line, which was derived from mesenchymal cells residing in murine fetal livers and accelerated the hepatic maturation of both murine hepatic progenitor cells and murine ESCs. In this study, hESC-derived AFP-producing cells were isolated by using a flow cytometer and co-cultured with MLSgt20 cells. The co-cultured hESC-derived AFP-producing cells had the immunocytological characteristics of hepatocytes, expressed mature hepatocyte markers (as indicated by reverse transcription and the polymerase chain reaction), and displayed higher hepatocyte functions including ammonia removal, cytochrome P450 3A4/7 activity, and the ability to produce and store glycogen. However, the MLSgt20 cells did not directly cause undifferentiated hESCs to mature into hepatocyte-like cells. The co-culture method was thus successfully shown to induce the differentiation of hESC-derived endodermal cells into functional hepatocyte-like cells.
論文 | ランダム
- Development of Plasmin-Selective Inhibitors and Studies of Their Structure-Activity Relationship
- Amino Acids and Peptides. LIII. Synthesis and Biological Activities of Some Pseudo-Peptide Analogs of PKSI-527,a Plasma Kallikrein Selective Inhibitor : The Importance of the Peptide Backbone
- Design of Bifunctional Inhibitors for Enzymes in Coagulation-Fibrinolysis System : Synthesis of Plasmin Inhibitor and Evaluation of Their Inhibitory Effect
- Design of Plasma Kallikrein Inhibitors : Functional and Structural Requirements of Plasma Kallikrein Inhibitors
- Purification Method for Human Plasma Kallikrein by a New Affinity Chromatography