[原著]Establishment of a Cell Line Derived from Human Osteosarcoma
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概要
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Establishment of the cell line ( HOS-O ) of osteosarcoma was successfully accomplished. The materials for this study were obtained from surgically removed tumor mass metastasized to the lung The patient was a 10 year-old girl, suffering from an original tumor in the right distal femur. The tissue culture has been started in September 1977, and the cell line has been maintained in a good condition for about five years. The shape of cultured cells is elongated-spindle and its chromosome number is 46. By histochemical examination, the cells revealed weakly positive at alkaline phosphatase, and moderately positive at acid phosphatase, and highly positive at nonspecific esterase and 5'-nucleotidase. Each of twice transplantations of those cells, to the back of nude mice, was successful. Histological features of the trasplanted tumors were resembled to that of the original sarcoma, showing a remarkable formation of osteoid tissue.
- 琉球大学医学部の論文
琉球大学医学部 | 論文
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