Bioelectrocatalytic current based on direct heterogeneous electron transfer reaction of glucose oxidase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode surfaces
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Multi-walled carbon nanotubes (MWCNTs) were synthesized on platinum plate electrodes bythe chemical vapor deposition (CVD) method. The MWCNTs synthesized on the Pt plate(MWCNTs/Pt) electrode were immediately immersed into solutions of glucose oxidase (GOX) to immobilize these enzymes onto the MWCNTs/Pt electrode surfaces. After the GOX was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current was increased from ca. -0.45 V (vs. Ag/AgCl/sat'd KC1), which was close to the redox potential of flavin adenine dinucleotide (FAD) as a prosthetic group of GOX under physiological pH values.
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