2次元電気泳動法による3倍性ギンブナ卵のタンパク質解析

概要

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To investigate the mechanisms of gynogenetic development of ginbuna, Carassius auratus langsdorfii, the protein constitution of growing oocytes, full-grown oocytes, matured eggs and fertilized eggs obtained from triploid ginbuna and diploid funa were analyzed by two-dimensional gel electrophoresis. The results are as follows ; (1) the one polypeptide which exists in bisexual diploid funa and exceptional triploid ginbuna is lacked in gynogenetic triploid ginbuna which does not decondense the penetrated sperm chromatin to male pronucleus; (2) the alteration of protein constitution following germinal vesicle breakdown in triploid ginbuna is different form that in diploid funa in quality; (3) number of polypeptides, distinctly detected in the polyacrylamide gel, slightly decreases after oocyte maturation in the diploid funa; (4) the protein constitution does not alter following fertilization. These results seem to suggest the hypothesis that the factor(s) of sperm chromatin decondensation is lacked in egg cytosol of gynogenetic triploid ginbuna and oocyte maturation in gynogenetic triploid ginbuna differ qualitatively from that in diploid funa.
信州大学理学部の論文
1984-10-15