ギンブナ卵ホモジェネートによる精子核クロマチンの拡散
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概要
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Sperm chromatin decondensing activity of funa eggs and oocytes was studied in vitro, in order to clarify the mechanism of sperm chromatin decondensation in egg cytoplasm. To assay for sperm chromatin decondensing activity, naked sperm nuclei treated with Triton were exposed to homogenate from oocytes and eggs. As a result of experiments using funa sperm, full-grown oocytes of diploid funa induced chromatin decondensation, though growing oocytes, matured eggs of diploid, and oocytes and matured eggs of triploid did not. On the other hand, using medaka sperm, fullgrown oocytes of triploid funa also induced chromatin decondensation. From the present study, it becomes apparent that homogenate from full-grown oocytes before germinal vesicle breakdown possess the ability to decondense chromatin. And it seems to be probable that egg cytoplasm of triploid ginbuna does not lack the ability to decondense chromatin but possesses the factor(s) inhibiting decondensation of sperm chromatin of the other sub-species.
- 信州大学理学部の論文
- 1984-10-15
信州大学理学部 | 論文
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