Studies on Bluing Effect in the Petals of Red Rose, IV. : Calcium in the Blue Spherical Body.
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Both the localization of calcium within the blue spherical body presented in the upper epidermal cells, and the role of the element in this body were studied. The upper epidermis of the bluing petals of a rose cultivar, cl-Crimson Glory, was fixed with 10 % neutral buffered formalin, and paraffin sections were prepared. The region corresponding to the central layer of the spherical body was clearly stained red with alizarin red sulfonate under pH 5.5. In the bluing petals treated with EDTA, the central layers of the bodies were not found and the body looked somewhat ring-like. These results show that the calcium localizes within the central body, (a) making a salt or being weakly bound with a tannic substance within the body, and (b) playing a role in making the layer rather rigid.
- 信州大学理学部の論文
- 1976-08-25
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