[原著]長期継代マウス骨肉腫培養細胞の継代数と移植部位による造骨能の比較検討

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Even after a long period of the passages, up to 3 years and 7 months, the established cell line derived from naturally occurred bone tumor in a NZB mouse still has the abilities of bone formation. The cell develops a tumor with variable degree of ossification by transplantation j.o the same strain mice. We studied the frequencies of the appearance of bone formation in the transplanted tumors histopathologically. Results obtained are as follows: At the group of intraperitoneal transplantation, the ratio of success transplantation case was 100%, higher than that of the dorsal subcutaneous transplantation group (82.6%). Simultaneously, we observed a tendency of more frequent appearance of the bone forming abilities at the intraperitoneal tumor, in spite of a short period. As to the average of survival time, the group transplanted on the back subcutaneously was 80.8 days and that of intraperitoneally transplanted group was about the half (42.7 days). Moreover, the abdominally transplanted tumors revealed bone or osteoid formation about 63%, while the back subcutaneous tumors were only 34.8%. We have tried the direct transplantations into the ventricular space of the heart or the heart or the tail-vein of mice. The rate of success cases by those transplantation were low and each were 75% and 54.5% respectively. We found that those works are so difficult technically and not suitable methods for an experiment deals with bone sarcomas. On the other hand, we experienced a high rate of metastases (50%) in the group which was transplanted subcutaneously on the back. From those fact we presumed that the dorsal subcutan might be a suitable site for transplantation concerning to the study of metastasis of bone tumors.
琉球大学医学部,Faculty of Medicine, University of the Ryukyusの論文

[原著]長期継代マウス骨肉腫培養細胞の継代数と移植部位による造骨能の比較検討

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