蛍光増白剤の影響-2-大腸菌による感受性試験およびサルモネラ菌による突然変異誘起性試験

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Following the previous paper, influences of fluorescent whitening agents (FWAs) on bacteria were examined. An experimental study was described with following results.(1) A paper disc spot method was performed on sensitivity test.Though Escherichia coli Try^- hcr^+, if anything, presented sensitive compared with E. coli Try^- her^- to coumarin (COU) and methylumbelliferone (MUM) containing paper discs (0.01-1.4 mg/disc), the significant correlations between two mutant strains and sensitivity to FWAs was not recognized. Sensitivity was hardly detected under diaminostilbene disulfonic acid (DDA).No appearance of revertant colonies (Try^- →Try^+) in E. coli on minimal medium plates was considered, at least, FWAs used in this test were not mutagens of the type of base change mutation.(2) Mutagenicity test without S-9 Mix showed that DDA and MUM presented weak positive histidine reverse mutation (His^- → His^+) in Salmonella typhimurium TA 98, and only DDA presented positive in S. typhimurium TA 100. And according to active reversion induced in S. typhimurium TA 98 under COU conditions seemed that COU was probably a chemical substance of a frameshift mutagen.