ラット腹腔肥満細胞に関する研究第1報 : 肥満細胞のpurification について

概要

論文の詳細を見る
To get a high recovery rate of mast cells from the rat peritoneal cavity, a new modified method was discussed using BSA density gradient. Three procedures, peritoneal lavage, washing of peritoneal cells and purification by BSA, were tested in this study, and the numbers of total cells and mast cells in each procedure were calculated. The recovery rate was the highest in BSA density gradient purification of the cells washed once with low speed centrifugation. The recovery rate was 73.7% and the number of mast cells taken from one rat was approximately 1.5×10(6). The purity of the cells with this procedure was 95.0% (range from 93.0 to 96.0%).
岡山大学温泉研究所の論文
1982-03-25