気管支喘息における抗原特異的 T cell clone の樹立とサイトカイン産生能に関する研究

概要

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To clarify the pathogenesis of bronchial asthma, we establishied T cell clones specific for Candida antigen and for mite antigen from the peripheral blood of three asthmatic patients. Flow cytometric analysis was performed on these clones. The surface phenotype of all clones was helper/inducer type. These clones were analyzed for the ability to produce interferon gammma (IFN-γ) and interleukin 4 (IL-4). The T cell clone established from tne patient who showed late asthmatic response (LAR) after bronchial inhalation of Candida, as well as the clones from the patient who showed IAR and LAR after inhalation of housedust mite, produced a higher amount of IFN-γ and lower amount of IL-4. The T cell clone established from the patient who were under corticosteroid therapy and showed IAR after inhalation of Candida produced lower amounts of IFN-γ and IL-4, which seemed to be inhibition of cytokine production from antigen specific T cell clone by corticosteroids. These findings indicate that T cell clones established from the patients who showed LAR produced a large amount of IFN-γ, suggesting that IFN-γ plays an important role in allergic reactions of LAR.