Engineering Bone Formation from Human Dental Pulp- and Periodontal Ligament-Derived Cells.
スポンサーリンク
概要
- 論文の詳細を見る
The original publication is available at www.springerlink.comA robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In this study, a method for generating bone tissue in vivo from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, beta-glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human bone morphogenetic protein 2 (rhBMP2) to osteogenic medium remarkably increased ALP activity and in vitro calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of osteocalcin, osteopontin, and dentin matrix protein 1 mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation in vivo when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility.
- 2010-07-08
論文 | ランダム
- 単一神経細胞を用いたシナプス開口放出の解析
- 中国の経済体制改革の論理と課題 (転換する中国経済)
- Sub-100fs Higher Order Soliton Compression in Dispersion-Flattened Fibers(Special Issue on Ultrafast Optical Signal Processing and Its Application)
- 成人看護学実習に対する学生の授業評価 : 教員の指導体制の変更による影響
- D-17 神経突起末端部のイオンチャネル(一般口演,第23回杏林医学会総会)