Steroid degradation genes in Comamonas testosteroni TA441: Isolation of genes encoding a Delta4(5)-isomerase and 3alpha- and 3beta-dehydrogenases and evidence for a 100kb steroid degradation gene hot spot.
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In previous studies, we identified two major Comamonas testosteroni TA441 gene clusters involved in steroid degradation. Because most of the genes included in these clusters were revealed to be involved in degradation of basic steroidal structures and a few were suggested to be involved in the degradation of modified steroid compounds, we investigated the spectrum of steroid compounds degradable for TA441 to better identify the genes involved in steroid degradation. TA441 degraded testosterone, progesterone, epiandrosterone, dehydroepiandrosterone, cholic acid, deoxycholic acid, chenodeoxycholic acid, and lithocholic acid. The results suggested TA441 having 3alpha-dehydrogenase and Delta4(5)-isomerase, and 3beta-,17beta-dehydrogenase gene, we isolated these genes, all of which had high homology to the corresponding genes of C. testosteroni ATCC11996. Results of gene-disruption experiments indicated that 3beta,17beta-dehydrogenase is a unique 3beta-dehydrogenase which also acts as a 17beta-dehydrogenase in TA441, and there will be at least one more enzyme with 17beta-dehydrogenating activity. The 3alpha-dehydrogenase and Delta4(5)-isomerase genes were found adjacent in the DNA region between the two main steroid degradation gene clusters together with a number of other genes that may be involved in steroid degradation, suggesting the presence of a steroid degradation gene hot spot over 100kb in size in TA441.
- 2010-06-08
論文 | ランダム
- 目録
- 最近の粒径測定装置 (特集:粉粒体プロセスにおける計測技術)
- 粉体の物性測定装置
- レ-ザ-ドップラ-法による粒径と帯電量の同時測定 (〔粉体工学会〕第29回夏期シンポジウム--粉体のキャラクタリゼ-ション)
- 博物館だより 台東区立書道博物館