イネ脂質代謝経路上のmutator誘発突然変異

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継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University水稲品種銀坊主種子のγ線照射後代に見いだされた突然変異誘発造伝子mutatorは、上記γ線照射で誘発された1細粒遺伝子slgの正常粒遺伝子(＋)への復帰突然変異に伴って活性化し、形態的あるいは生理的形質に種々の変異を誘発する。しかし、植物体構成物質の成分についてはまだ突然変異誘発の有無が明らかにされていない。そこで、本研究では、生体膜を形成する重要な成分であり、すでに一部の代謝反応系が解明されている脂質に着目し、mutatorによって誘発された形態的あるいは生理的形質に関する突然変異固定型48系統を供試して、脂質代謝経路におけるmutator誘発突然変異の有無を探索した。まず、供試全系統について全脂質の脂肪酸組成を調査したところ、組成脂肪酸の含有比率が正規分布から有意に外れる9系統が見いだされた。ついで、これら9系統のうち解析に十分な量の種子が得られた6系統および上記9系統についで大きな変異を示した8系統計14系統について、種子中に多く含まれる3脂質分子種フォスファチジルセリン、フォスファチジルエタノールアミンおよびフォスファチジルコリンの脂肪酸組成を調査したところ、いずれの分子種についても、脂肪酸含有比率が原品種銀坊主と有意に異なる系統が相当数認められた、このことから,mutatorは植物体構成物質の成分に関する変異も誘発すると考えられた。　　（英文）　The mutator that was found out first in the M_3 progeny derived from a γ-ray irradiated M_1 seed of a japonica rice variety Gimbozu, causes novel mutations in many different loci for morphological and/or physiological traits, when activated by the reversion of a mutable slender-glume gene slg., which was induced by γ rays in the above identical M_1 seed. However, it has not yet been confirmed if the mutator also mutate the structure material of plants. Lipid is one of the most important components of biomembrane, and has partly been elucidated for metabolic pathway. In view of this situation, the mutations that might have been induced by the mutator on lipid metabolic pathway was screened through the analysis of the fatty acid composition of lipid contained in seeds, using 48 mutant lines with some mutator-induced morphological and/or physiological mutations. First, the 48 lines were analyzed for their respective fatty acid composition in total lipid, and nine of these lines each proved to be significantly apart from the population mean in at least one of the percent contents of 5 sorts of fatty acid. Then, 14 lines the percent contents of which largely deviated from the population mean, inclusive of 6 from the above 9 lines, were selected and analyzed for fatty acid composition of 3 lipid molecular classes, phosphatidylserine, phosphatidylethanolamine and phosphatidylcholine. As a result, it was observed in all the classes that a considerable number of lines were significantly different from the original variety in the percent content of fatty acid. This may strongly suggest that the mutator induces mutations also in the components of structure material of plants.
近畿大学生物理工学研究所,キンキダイガクセイブツリコウガクケンキュウジョ,Kinki Daigaku seibutsurikogaku Kenkyushoの論文

イネ脂質代謝経路上のmutator誘発突然変異

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