[原著］P815 Tumor Cells Express an Intrinsic Absolute Dominant Tumor Specific Transplantation Antigen (TSTA) That Functions in Immune Regulation

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As we have reported^1) , Thy.^- bone marrow cells passed through nylon wool (NW-BM) augmerited the generation of cytotoxic T lymphocytes (CTL) against minor histocompatibility (mH) antigens in primary mixed lymphocyte cultures (MLC). This study showed that when DBA/2 (H-2^d) responder lymph node (LN) cells were stimulated with syngeneic P815 (H-2^d , DBA/2 origin) tumor cells in the presence of DBA/2 helper NW-BM cells, the CTL specific for the tumor specific transplantation antigen (TSTA) of P815 tumor cells were induced easily in primary cultures in vitro. We also showed that the CTL from Balb/c (H-2^d) or B10D2 (H-2^d) responder cells stimulated with DBA/2 spleen cells killed DBA/2 Con A blast cells but not P815 tumor cells. Since the CTL recognized the absolute dominant mH antigen of DBA/2 lymphoid cells according to the previous report^1) P815 tumor cells had lost this antigen. On the other hand, the CTL from Balb/c or B10D2 responder cells stimulated with allogeneic P815 tumor cells lysed P815, but not DBA/2 target cells. Thus, P815 tumor cells expressed the intrinsic absolute dominant antigen, which was absent in DBA/2 lymphoid cells.