[原著］Amino Acid Sequence of Avian Hemoglobins and Its Evolutiomary Implication at仙e Molecular Level 1. Partial Amino Acid Sequence of the $ \alpha $ Chain of Japanese Quail QII Hemoglobin

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Amino acid sequence of the $ \alpha $ chain of Japanese quail (coutournix coutournix japonica) hemoglobin QII was investigated and determined except its 'core' portion. The carboxymethylated $ \alpha $ chain was hydrolysed with trypsin and 13 tryptic peptide fragments were purified by using ion exchange chromatography, Sephadex gel filtration, etc. Ten peptides of them, T-1, T-2, T-3, T-5, T-7, T-8, T-10, T-11, T-12 and T-13 were short ones and their amino acid sequence was directly determined mainly by Edman degradation method. Since peptides T-4 and T-6 were longer ones, they were further hydrolysed with chymotrypsin and the amino acid sequence of the chymotryptic peptides purified was respectively determined by DNS-Edman or Edman degradation method. The peptide T-9 was also a longer one and was subjected to digestion with thermolysin and the amino acid sequence of the thermolytic peptides purified was determined by Edman degradation method. The amino acid sequence of the soluble fraction of the $ \alpha $ chain of Japanese quail QII hemoglobin thus determined was compared to that of chicken All hemoglobin. Their homology was so high with each other that the percentage of amino acid differences was 10.9 and the amino acid residues placed in the important position involved in hemoglobin function was less variable.
琉球大学医学部の論文