[原著］Beneficial Effect of Oxygen-Enriched Medium for Isolation of Mitochondona

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We compared oxidative phosphorylation activities and ultrastructure of mitochondria isolated with a normal isolating medium and with an oxygen-enriched medium. RCI. Q0_3, Q0_4 and OPR values of mitochondria isolated with the oxygen-enriched solution were 14 - 34% high as compared with those isolated with the normal medium, when succinate was employed as a substrate. Electron microscopic study showed that the ratio of small size of mitochondria (< 1 $ \mu $m) was 87 % in the total numbers of the fraction isolated with the oxygen-enriched medium, whereas 77 % in that isolated with the normal medium. The results suggest a possible role of oxygen-enriched isolating medium in reserving mitochondrial function and ultrastructural integrity, presumably due to protection of mitochondrial aging or disruption during isolation procedure.