Response of cellulase activity in pH-controlled cultures of the filamentous fungus Acremonium cellulolyticus.
スポンサーリンク
概要
- 論文の詳細を見る
Cellulase production was investigated in pH-controlled cultures of Acremonium cellulolyticus. The response to culture pH was investigated for three cellulolytic enzymes, carbomethyl cellulase (CMCase), avicelase, and beta-glucosidase. Avicelase and beta-glucosidase showed similar profiles, with maximum activity in cultures at pH 5.5-6. The CMCase activity was highest in a pH 4 culture. At an acidic pH, the ratios of CMCase and avicelase activity to cellulase activity defined by filter paper unit were high, but at a neutral pH, the beta-glucosidase ratio was high. The pH 6.0 culture showed the highest cellulase activity within the range of pH 3.5-6.5 cultures. The saccharification activity from A. cellulolyticus was compared to those of the cellulolytic enzymes from other species. The A. cellulolyticus culture broth had a saccharification yield comparable to those of the Trichoderma enzymes GC220 and Cellulosin T2, under conditions with the same cellulase activity. The saccharification yields from Solka floc, Avicel, and waste paper, measured as the percent of released reducing sugar to dried substrate, were greater than 80% after 96 h of reaction. The yields were 16% from carboxymethylcellulose and 26% from wood chip refiner. Thus, the A. cellulolyticus enzymes were suitable for converting cellulolytic biomass to reducing sugars for biomass ethanol production. This study is a step toward the establishment of an efficient system to reutilize cellulolytic biomass.
論文 | ランダム
- 外陰掻痒症 (産婦人科医に必要な皮膚疾患の知識) -- (私はこうしている(治療法を))
- ハリ麻酔の基礎的研究 : 2点識別法による麻酔効果の統計学的検討
- 7.某家内産業における低濃度有機溶剤作業の神経心理学的検査に及ぼす影響(第10回産業神経・行動学研究会)
- 産婦人科領域のMinor Operationへの針麻酔の応用
- アノーディック・ストリッピング・ボルタンメトリー法と原子吸光法の比較 : 気中、尿中鉛量の分析