Cryoimmunologic antitumor effects enhanced by dendritic cells in osteosarcoma
スポンサーリンク
概要
- 論文の詳細を見る
Background We previously reported a limb-salvage technique by treating tumor-bearing bone with liquid nitrogen. We also reported systemic antitumor immunity was enhanced by cryotreatment in a murine osteosarcoma (LM8) model. We therefore combined the cryotreatment of tumor with dendritic cells to promote tumor-specific immune responses. Questions/purposes We determined whether our technique could enhance systemic immune response and inhibit metastatic tumor growth in a murine osteosarcoma model. Materials and Methods To evaluate activation of the immune response, we prepared six groups of C3H mice (80 mice total): (1) excision only, (2) dendritic cells without reimplantation of the cryotreated primary tumor, (3) reimplantation of the cryotreated primary tumor alone, (4) dendritic cells combined with reimplantation of the cryotreated primary tumor, (5) dendritic cells exposed to cryotreated tumor lysates without reimplantation of the cryotreated primary tumor, and (6) dendritic cells exposed to cryotreated tumor lysates with reimplantation of the cryotreated primary tumor. We then compared and verified the activation state of each group's antitumor immunity. Results Mice that received dendritic cells exposed to cryotreated tumor lysates with reimplantation of the cryotreated primary tumor group had high serum interferon γ, reduced pulmonary metastases, and increased numbers of CD8(+) T lymphocytes in the metastatic areas. Conclusions Combining tumor cryotreatment with dendritic cells enhanced systemic immune responses and inhibited metastatic tumor growth. Clinical Relevance We suggest immunotherapy could be developed further to improve the treatment of osteosarcoma. © The Association of Bone and Joint Surgeons® 2010.
論文 | ランダム
- 22aYE-2 Possible ω bound states in nuclei produced with the (γ,p) reaction
- OSCE の評価者の違いによる評価の一致性に関する検討
- 22pYE-1 Kaon and Hyperon Photoproduction induced by Wess-Zumino-Witten term
- 22aYE-5 有限密度系におけるU_A(1)対称性の回復について(22aYE 中間子原子核・ハドロン構造,理論核物理領域)
- 朝日大学歯学部附属病院歯科医師臨床研修における初期研修についてのアンケート調査