Immunoadjuvant activity of crude lectin extracted from Momordica charantia seed

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The aim of this study is to investigate the immunoadjuvant activity of the crude Momordica charantia lectin (crMCL) extracted from seed using β-galactosidase (β-gal) as the model antigen. BALB/c mice were injected intramuscularly with β-gal alone or β-gal + crMCL for up to four immunizations at two-week intervals. After administration of 2 doses, the IgG-specific titer to β-gal was significantly higher in mice in the β-gal + crMCL group than in that from the animals from the β-gal alone group, while it was about the same in both groups after 1 dose. Our data suggest that crMCL may help raise antibodies under the prime and boost administration regimen and could be a potent vaccine adjuvant.ニガウリ種子由来粗精製レクチン(CrMCL)のアジュバント効果について,ベータガラクトシダーゼをモデル抗原として解析した.BALB/cマウスを抗原単独投与群と抗原+CrMCL投与群に分け,血清中の抗原特異的IgG抗体価を測定したところ,1回免疫では両群で差がなかったが,追加免疫で抗原+crMCL投与群が有意に高かった. CrMCLはプライムブースト投与法に有用なアジュバントであることが示唆された.
2008-05-25