DEAE-デキストラン共重合体を非ウイルス性遺伝子導入キャリアーとして用いた、培養細胞への外来遺伝子導入

概要

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Transfection efficiency of 2-diethyl aminoethyl-dextran-methyl methacrylate graft copolymer (DDMC) was evaluated with pGL3 DNA and COS-7 cells. Relative light unit (RLU) of luciferase proteins was measured using 96 hole-microtiter trays. Results were as follows: 1) When DNA (0.05 ㎍/well) and DDMC (4.0 ㎍/well) were transfected into the cells, RLU was about double higher than DNA and DEAE-dextran. However, it was ofequal or lower value when the less quantity of DDMC was used. 2) The first peak of RLU on DDMC and DEAE-dextran appeared at 72 hours and 48 hours after the transfection, respectively. The delay luciferaseexpression may be explained by the fact that the complex of DNA and DDMC in cells is hard to be decomposed.The complex will be formed by Michaelis Menten type equation such as complex amount = K1 (DNA concentration) (DDMC oncentration).