A Novel Fluorescent Sensor Protein for Visualization of Redox States in the Cytoplasm and in Peroxisomes.
スポンサーリンク
概要
- 論文の詳細を見る
神経疾患モデル細胞と正常細胞の酸化還元状態の違いを検知できるセンサータンパク質の開発に成功. 京都大学プレスリリース. 2010-05-25. http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2010/100525_1.htmReactive oxygen species are generated within peroxisomes during peroxisomal metabolism. However, due to technological difficulties, the intraperoxisomal redox state remain elusive and the effect of peroxisome-deficiency on the intracellular redox state is controversial. A newly developed, genetically-encoded fluorescence resonance energy transfer (FRET) probe, Redoxfluor, senses the physiological redox state via its internal disulfide bonds, resulting in a change in conformation of the protein leading to a FRET response. We made use of Redoxfluor to measure the redox states at the subcellular level in yeast and Chinese hamster ovary (CHO) cells. In wild-type peroxisomes harboring an intact fatty acid beta-oxidation system, the redox state within the peroxisomes was more reductive than that in the cytosol, despite the fact that reactive oxygen species were generated within the peroxisomes. Interestingly, we observed that the redox state of the cytosol of cell mutants for peroxisome-assembly, regarded as models for a neurological metabolic disorder, was more reductive than that of the wild-type cells in yeast and CHO cells. Furthermore, Redoxfluor was utilized to develop an efficient system for screening of drugs that moderate the abnormal cytosolic redox state in the mutant CHO cell lines for peroxisome assembly, without affecting the redox state of normal cells.
論文 | ランダム
- M9東北地方太平洋沖地震による東日本大震災と福島第一原発事故
- 地形学は東北地方太平洋沖地震を予測できたのか (緊急特集 東日本大震災)
- 平成23(2011)年東北地方太平洋沖地震に伴う高速道路の被災状況及び応急復旧概要
- 東北地方太平洋沖地震のダムへの影響--福島県南部
- 東日本大震災(東北地方太平洋沖地震)について(速報その3)