蓚酸酸化酵素を用いた尿中蓚酸定量法について - Gas-chromatography 法との比較 -

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1)蓚酸酸化酵素を用いた尿中蓚酸定量法(市山法)を検討した.変動係数は2.49±0.97 (S.D.)%,回収率は99.5±3.3 (S.D.)%であり,操作が簡便であるばかりでなく,非常に精度の高い方法であることが判明した.2)同一被検尿について,酵素法とgas-chromatography法を同時に測定した結果,両者に危険率1%以下の有意な相関関係があったUrinary oxalate is one of the most important constituents of urolithiasis, but the determination of oxalate in urine has not been performed as a routine laboratory examination. We tried to measure oxalate in urine by the enzymatic method with oxalate oxidase. The linearity of the standard curve and reproducibility of this method were confirmed. (Linearity: r = 0.996, S.D./mean: 0.5-3.6%, recovery rate: 99.5 +/- 3.3 (mean +/- S.D.)%) The correlation between this method and gas-chromatographic method was 0.926. The enzymatic method with oxalate oxidase can be utilized for determining the urinary oxalate as a routine laboratory examination.