ヒト尿路***悪性腫瘍のヌードマウスへの異種移植 - 移植成績と移植腫瘍の性状 -
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1981年6月より1984年5月までの3年間に6種40例のヒト尿路***悪性腫瘍をヌードマウスに移植し,その成績を検討した.40例中7例が生着し,うち3例に継代可能であった.腫瘍別の生着率は,腎腫瘍28.6%,膀胱腫瘍23.1%で他の組織型のものは生着しなかった.腫瘍組織の採取部位は,原発巣と転移巣で差は見られなかった.生着した7腫瘍のうち1年以上長期にわたり継代可能のものは腎細胞癌の3株のみであった.初代移植成功の7例で,移植より生着までの期間をみると,最短は腎細胞癌の12日,最長は膀胱腫瘍の36日で平均24.4日であった.生着率と移植に要した時間の関係では,3時間以内29.4%,3～5時間18.2%で,可及的短時間内での移植が望まれた.抗asialo GM1抗体投与によってとくに生着率に影響は及ぼさなかった.初代移植成功の7腫瘍の病理組織学的検討では,腎細胞癌4例はいづれも組織学的異型度が高度で,かつ転移を有する進行癌のため術後早期に死亡した.膀胱腫瘍も生着3例中2例は低分化型腫瘍であり,腫瘍の分化度,増殖能と可移植性との関連性が示唆された.長期継代可能な腎細胞癌3株の増殖態度をみると,各株により増殖速度が異なるが,各々継代7代目,9代目,10代目以降になると,ほぼ安定した増殖を示した.移植腎細胞癌3株の原腫瘍と移植継代腫瘍の組織像を比較検討した.うち2株では継代により間質の減少傾向がみられ,medullaryな性格が強くなっているが,3株とも原腫瘍の基本的な組織学的特徴をよく維持していたBetween July, 1981 and June, 1984, 40 malignant urogenital neoplasms were heterotransplanted into nude mice to clarify the biological characteristics of malignancies and screen the effective anticancer drugs against them. The primary tumor take was confirmed in 7 tumors (17.5%), including 4 of 14 renal cell carcinomas (28.6%) and 3 of 13 bladder cancers (23.1%). No primary take was recognized in renal pelvic, ureteral, prostatic and testicular cancers. Three of the 7 tumors (43%) with primary take (7.5% in total) have been serially transplanted for more than a year. These three established tumors were all renal cell carcinomas and were designated as AM-RC-1, AM-RC-6 or AM-RC-11. The take rate of tumor tissues obtained from primary and metastatic lesions was 18.2% (6/33) and 14.3% (1/7), respectively. The duration from tumor inoculation to take was examined in seven transplantable tumors. The shortest was 12 days in renal cell carcinoma, the longest was 36 days in bladder cancer and the average was 24.4 days. The correlation between the time from tumor extraction to inoculation and transplantability was investigated. The take rate was 29.4% within 3 hours, and 18.2% beyond 3 hours. The obvious effect of anti-asialo GM1 antibody on tumor take, which inhibits the natural killer activity of mice or rats selectively, was not demonstrated owing to the small number of cases. The histopathological features of seven original tumors with primary take were examined. All of the 4 renal cell carcinomas were shown to be histologically high grade and advanced. Two of the 3 bladder cancers were poorly differentiated. Therefore, the differentiation or growth rate of the tumor was suggested to be closely correlated with transplantability. The three strains of renal cell carcinomas transplanted serially for a long-term period, AM-RC-1, AM-RC-6 and AM-RC-11 disclosed a constant growth after the 7th, 9th and 10th inoculation respectively. The renal cell carcinomas serially transplantable to nude mice maintained the basic histologic findings of the original tumor even after long-term serial transplantation.