Expression of recombinant sea urchin cellulase SnEG54 using mammalian cell lines
スポンサーリンク
概要
- 論文の詳細を見る
We previously identified the cellulase SnEG54 from Japanese purple sea urchin Strongylocentrotus nudus, the molecular mass of which is about 54 kDa on SDS-PAGE. It is difficult to express and purify a recombinant cellulase protein using bacteria such as E. coil or yeast. In this study, we generated mammalian expression vectors encoding SnEG54 to transiently express SnEG54 in mammalian cells. Both SnEG54 expressed in mammalian cells and SnEG54 released into the culture supernatant showed hydrolytic activity toward carboxymethyl cellulose. By using a retroviral expression system, we also established a mammalian cell line that constitutively produces SnEG54. Unexpectedly, SnEG54 released into the culture medium was not stable, and the peak time showing the highest concentration was approximately 1 to 2 days after seeding into fresh culture media. These findings suggest that non-mammalian sea urchin cellulase can be generated in human cell lines but that recombinant SnEG54 is unstable in culture medium due to an unidentified mechanism.
- 2010-05-07
論文 | ランダム
- Limit theorem related to an interface of three dimensional Ising model
- 熱電対の特性を見なおそう その原点からの考察 (温度計測(特集))
- 気道抵抗(Rint) (特集 これだけは知っておきたい気道疾患の最新診断検査法) -- (生理学的検査)
- P-43 物理的現象に対する物質の性質の付与 : 熱と音の比較(ポスター発表)
- 外国語科目「英語」の改善に向けて--四つの提言