Expression of recombinant sea urchin cellulase SnEG54 using mammalian cell lines
スポンサーリンク
概要
- 論文の詳細を見る
We previously identified the cellulase SnEG54 from Japanese purple sea urchin Strongylocentrotus nudus, the molecular mass of which is about 54 kDa on SDS-PAGE. It is difficult to express and purify a recombinant cellulase protein using bacteria such as E. coil or yeast. In this study, we generated mammalian expression vectors encoding SnEG54 to transiently express SnEG54 in mammalian cells. Both SnEG54 expressed in mammalian cells and SnEG54 released into the culture supernatant showed hydrolytic activity toward carboxymethyl cellulose. By using a retroviral expression system, we also established a mammalian cell line that constitutively produces SnEG54. Unexpectedly, SnEG54 released into the culture medium was not stable, and the peak time showing the highest concentration was approximately 1 to 2 days after seeding into fresh culture media. These findings suggest that non-mammalian sea urchin cellulase can be generated in human cell lines but that recombinant SnEG54 is unstable in culture medium due to an unidentified mechanism.
- 2010-05-07
論文 | ランダム
- プランニングから見た人工地盤緑化技術の問題点 (特集 建物と緑,技術の最前線)
- アクロス福岡のステップガ-デン (特集 緑--人間・建築・都市とのふれあい)
- 「らんの里 堂ヶ島」について(平成6年度日本造園学会賞受賞者業績要旨)
- 伝熱変換装置に就て
- 247 熱平衡を考慮した連続加熱炉の伝熱計算(形鋼の二次加工・加工性・新製造法・熱処理・ダイス寿命・炉の制御, 加工, 日本鉄鋼協会 第 97 回(春季)講演大会)