非淋菌性尿道炎に於けるPleuropneumonia-LikeOrganisms(PPLO)に関する知見補遺第II編PPLOの病原性に関する実験的研究 I.PPLO人尿株の艀化鶏卵内培養 II.PPLO人尿株のマウス皮下接種実験 III.PPLO人尿***株の猿の尿道内接種実験

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1) The cultivation of PPLO in the developing chick embryo. Three strains of PPLO isolated from the human g enitourinary tract were cultivated in. the developing chick embryo ; two strains had been isolated from non-gonococcal urethritis in the male and one strain ("Campo L") isolated in America. Seven day old embryos were used for the yolk sac and the chorioallantois cavity routes of inoculation. All 3 strains grew well in the chick embryo and they were gr o wn consectively for 9. transfers when they were inoculated by the yolk sac route. No increase in motality or no pathological involvem ent could be observed in the infected chick embryo. 2) Subcutaneous inoculation of PPLO in mice. Seven strains of PPLO were examin e d to determine their pathogenicity for mice ; & strains had been isolated from non-gonococcal urethritis in the male and 1 the "Campo L" strain. Cultures of each of these strains were subcutaneously inoculated in mice. All except 1 of the 7 strains did not affect mice after subcutaneous inoc u lation. Only the "Campo L" strain produced local abscesses in mice by injection into the foot pads. 3) Intra-urethral inoculation of PPLO in monkeys (Macacus irus). One strain of PPLO isolated from non-gonococcal urethrit i s (acute form) in the male was inoculated intra-urethrally into two male monkeys (Macacus irus) without any subsequent clinical or bacteriological evidence of infection.