尿石溶解の研究(第4報) 尿石の非晶型有機成分の化学的分析
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For the purpose of the therapeutic dissolution of urinary calculi it seems to be very necessary to clarify the organic components of them, the study of which has been scarcely reported. The organic components reported have been analyzed only by the methods of elementary analysis. Recently it's histochemical study has been also reported. The present paper reports the results of the qualitative analytical studies for the amorphous organic components of urinary calculi by the methods of biochemical analysis. 45 g of mixed powder of urinary calculi (from bladder 3, kidney 1 and mixed pieces of various calculi which were used for the previous experiments but the origin of them remains unknown) was extracted, each in a separated fraction, by agents in the following order successively: petroleum ether, ether, chloroform, alcohol, cold water, hot water, 1 % aqueous solution of HCl, 5 % that of NaOH and 5 % that of HC!. Then each of the extracts was treated by some chemical procedures such as distillation, vacaum distillation, neutralization, salting out and hydrolysis with HCI solution. All of these fractions were analyzed by usual biochemical qualitative methods as below. Tests for protein and amino acid: ninhydrin test. biuret test. Millon's test. Hopkis-Cole's test. Sakaguchi's test. Molisch's test. precipitation test by lead acetate, ferrocianide, picric acid, phosphotungustic acid, sulfosalicylic acid and alcohol. heat coagulation. saltion out by ammonium sulphate. Tests for carbohydrate: Benedict's test. Nylander's test. orcin-HCI test. diphenylamine test. Reduction test by Fehling's reagent. naphthoresorcin test. cysteine-H2S04 test. Tests for lipoid: acrolein test. Salkowsky's test. precipitation test by cadmium chloride. Lieberman- Burchard's test. Tests for other components: Ehrlich's aldehyde reaction. Obermyer's test. Jaffe's test. murexide test. Diazo reaction. Schledinger's test. precipitation test by mercury acetate. color develop meant by ferric chloride. Weiss's test. combustion. test by adding NaOH or acetic acid. test of P and S after hydrolysis by acid. Next, for protein, polysaccharide (using the extracting method of Sauda and Egami's technique) and nucleic acid (using the Levene's extracting method) in the powder was specifically analyzed. As the results of the above tests it was detected that urinary calculi have amino acid, protein, derived protein, mucopolysaccharide with and without S04, lecithin-like substance, animal gum-like substance and urinary pigment as the amorphous organic components.
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