タツナミガイ体壁網状筋の興奮収縮連関と収縮–弛緩に伴う細胞内Ca 動態に関する生理学および細胞化学的研究
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To clarify the excitation-contraction coupling and the intracellular Ca movementduring the contraction-relaxation cycle in a molluscan smooth muscle, the reticular bodywall muscle (RBWM) of Dolabella auricularia, physiological experiments on the mechanicalresponse, ultrastructural observations and Ca cytochemistry using the pyroantimonate(PA) method were carried out. The plasma membrane of RBWM fibers frequently showedtubular invaginations, closely resembling the transverse tubules (T tubule) of skeletal muscle. Numerous dyadic structures were found just beneath the plasma membrane, suggesting the Ca release from SR during contraction. The development of K-induced contracture tension was remarkably repressed by the removal of external Ca or by the application of Mn ions (10 mM). Caffeine (1 mM) could not cause the development of isometric tension in the Ca-free solution,while rapid cooling of the fiber bundle induced a significant magnitude of tension rise. In the relaxed fibers, the PA precipitate was found in the SR and the inner surface of the plasma membrane, indicating the intracellular Ca localization. In contacting fibers, the PA precipitate was diffusely distributed in the myoplasm, with corresponding precipitate decrease at the peripheral structures. These results indicate that the RBWM fibers are activated mainly by the inward movement of extracellular Ca, although the contribution of Ca released from the intracellular storage structures can not be negligible.
- 2009-06-30
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