感染細胞DNAの制限酵素切断によるアデノウイルス同定　第1報　Ethidium Bromide 染色

概要

論文の詳細を見る
The identification of adenoviruses by cleavage patterns with restriction endonucleases was presented. The results obtained are as follows ; 1) Adenovirus type 7 (Ad7) (strain Grider) DNA and infected cell DNA were cleaved with restriction endonuclease HindIII, BamHI, SalI and EcoRI, and fractionated by agarose gel electrophoresis. Cleavage patterns of adenovirus DNA could be clearly observed in the presence of infected cell DNAs. 2) SalI, HindIII and EcoRI cleavage patterns of Ad7 (strain Grider) DNA were identical with or very similar to those of Ad7 (strain Gomen) DNA but the differences among these DNAs were found clearly when BamHI was used for cleavage. 3) Cleavage patterns of Ad3 (strain G.B) and Ad7 (strain Grider) infected cell DNAs with HindIII, BamHI, SalI and EcoRI were studied. By cleavage with BamHI, we could distinguish between Ad3 and Ad7 DNAs. And each DNA from adenoviruses group A (Adl2 and Ad31), B (Ad3 and Ad7), C (Ad2 and Ad5), D (Ad9 and Ad13) and E (Ad4) had a specific cleavage pattern with BamHI, distinguishable from each other. These results indicate that we can identify adenovirus strains by the cleavage patterns with one or few suitable restriction endonucleases using DNA from a small amount of infected cells.
札幌医科大学の論文
1981-10-01