エンドトキシン血症の実験的・臨床的研究
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The Limulus test is a useful and highly sensitive method for detecting endotoxin. Lysate which was extracted from the amebocytes of Tachypleus tridentatus showed almost the same reactivity for endotoxin, as described for Limulus polyphemus. The results obtained in the present experimental and clinical application of the Limulus test are follows : 1. The lysate from Tachypleus tridentatus was capable of detecting endotoxin of as little as nanogram per ml. 2. The gel-formation activity of the lysate from each Tachypleus, each sampling lot, and each kind of endotoxin was different. The lysate was sensitive to Lps B (Lipopolysaccharide B) Serratia marcesc, 250-1,000 times more sensitive compared to Lps B E. coli 0127: B8. 3. The lysate gel-formation activity of the endotoxin was compared to its biological activity using LD50 with Actinomycin-D treated mice. No significant correlation was observed between the gel-formatin activity and biological activity of three Lps B, Serratia marcesc, E. coli 0127 : B8 and E. coli 026 : B6. 4. The plasma Lps B was more rapidly cleared in the rats administered Lps B Serratia marcesc. 25μg/100g b.w. than in those given 200μg. It was found that about 40% of the injected dose was recovered with the Limulus test in the plasma, liver, spleen, kidney and lung at 3hrs. after administration of 200μg/100g b.w. The liver contained more than 35%, while the other four organs contained an additional 5% of recoverable Lps B. However, Lps B in the liver decreased to 3% or so after 24hrs. 5. Endotoxemia developed in rabbits after occlusion of the superior mesenteric artery. The homogenates of lung and liver revealed extremely high concentrations of endotoxin than did the spleen or kidney after the release of occlusion, with the lung especially containing much more endotoxin than observed in the rats administered intravenously. 6. Fifty-eight cases of clinical endotoxemia were demonstrated with the Limulus test in 121 patients who had abdominal surgical diseases. Results of the test were often positive, especially in cases of obstructive jaundice, peritonitis or intraperitoneal abscess. Shock and thrombocytopenia were observed in many of these patients as well as symptoms of infection; namely, fever, tachycardia or leucoeytosis, etc. Their mortality rate was 72.4%.
- 札幌医科大学の論文
- 1980-12-01
札幌医科大学 | 論文
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