山羊の乳腺および乳管洞上皮の微細構造について
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泌乳期,成熟処女期,乾乳期の山羊における乳腺および乳管洞(乳槽)乳腺部の上皮細胞を観察し次の所見を得た. 1. 泌乳期の腺胞では大型の腺細胞が単層に配列し,拡張した腺腔内には脂肪滴などの分泌物が多量含まれている. 腺細胞の遊離面には少数の短いmlcrovilliが認められ,基底膜に接する基底面には basal infolding がよく発達し, 豊富な細胞質には脂肪滴, カゼイン空胞のほか, r-ER, Golgi 装置, mitochondria などがよく発達している.カゼイン粒子はr-ERおよびGolgi装置で形成され, おもに reverse pinocytosis によって腺腔へ放出されるようであり, 脂肪滴は decapitation によって細胞体から分離放出される. 2. 処女期および乾乳期の腺胞では立方状の腺細胞が集合して認められ,貧弱な細胞質中には tonofi1aments が豊富に存在し, r-ER, free-ribosome, mitochondria, 限界膜を持った danse bodies, 脂肪滴などが少数認められる. なお, 乾乳期の乳腺細胞には種々な形態および大きさの1ysosome様小体がかなり多数認められる. 3. 乳管洞上皮細胞は2～3層に配列し,乾乳期のものでは同時期の乳腺細胞に見られたと同様の1ysosome様小体がしばしば認められる. このことを除けば, 泌乳期, 処女期, 乾乳期を通じて,その微細構造に大差は認められず,処女期および乾乳期の乳腺細胞の構造とよく類似している.The goat's mammary gland and the gland cisterns of udder cisterns were examined by light and electron microscopy. The specimens from 3 lactating, 1 adult virgin and 1 involuted dry phase female were fixed in ice-cold 1 or 2% OsO_4 buffered with s-Collidine at pH 7.4. After 2-3 hours of fixation, the tissue blocks were dehydrated in a series of graded ethanol, and embedded in Epon 812. Ultrathin sections for electron microscopy were stained with uranyl acetate and lead citrate, and examined in a Hitachi HU-11 D-S electron microscope. For light microscopy, thicker sections from the same blocks were stained with toluidine blue. The observations are summarized as follows: 1. In lactating mammary alveoli, large glandular cells appear as a single layer. The alveolar lumens are distended with milk. A few small microvilli are present at the luminal surfaces of the secretory cells, and the basal surfaces along the basement membranes show well developed basal infoldings. The cytoplasm has many lipid droplets, casein vacuoles, r-ER, mitochondria and well developed Golgi apparatus. The casein particles seem to be formed at r-ER and the Golgi apparatus and discharged into the lumen by means of reverse pinocytosis. The lipid droplets are released by way of the decapitation. 2. In the resting udder from an adult virgin or an invoulted dry phase female, cuboidal gland cells gather up to form the alveoli. Although the acinal cells are rich in distinct tonofilaments which are scattered throughout the poor cytoplasm, but contain small numbers of r-ER, free-ribosome, mitochondria, lipid droplets and dense bodies. The cells of involuted dry phase, besides, have a considerable number of various lysosomal bodies. 3. The gland cisterns of udder cisterns are surrounded by 2 or 3 lyers of sinus epithelial cells. In the whole examination, the sinus epithelial cells appeared to have no clear distinction of their fine structure and bore a resemblance to the acinal cells of the adult virgin or the involuted dry phase female, but the sinus cells of the involuted dry phase again have a considerable number of various lysosomal bodies.
- 宮崎大学農学部の論文
- 1972-10-00
宮崎大学農学部 | 論文
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