Characterization of the mechanism of Zidovudine uptake by rat conditionally immortalized syncytiotrophoblast cell line TR-TBT
スポンサーリンク
概要
- 論文の詳細を見る
著者最終稿を登録可能.Purpose. To characterize the uptake mechanism of zidovudine (AZT), a nucleoside reverse transcriptase inhibitor, in syncytiotrophoblast cells using the TR-TBT 18d-1 cell line previously established by our group. Materials and Methods. The effects of several transporter inhibitors on the initial and steady-state apical uptake of AZT by TR-TBT 18d-1 were characterized, in order to identify the transporter(s) involved. Results. Initial uptake of AZT was sodium-independent and saturable; the K m value was about 16 μM. Nitrobenzylthioinosine (NBMPR), probenecid and cimetidine each had little effect on the saturable AZT uptake, indicating that well characterized transporters, such as organic anion transporters (OATs and OATPs), organic cation transporters (OCTs) and equilibrative nucleoside transporters (ENTs), are not involved. However, thymidine and 2′-deoxyuridine strongly inhibited AZT uptake. These results suggest that an unidentified nucleoside uptake transporter is responsible for the uptake of AZT. Cyclosporin A, Ko143 and probenecid had little effect on AZT accumulation by TR-TBT 18d-1 cells, suggesting that transporter-mediated efflux of AZT is not substantial. Conclusion. Our results indicate that saturable AZT uptake into TR-TBT 18d-1 is mediated by a so-far-unidentified transporter. © 2008 Springer Science+Business Media, LLC.
論文 | ランダム
- 公衆通信回線による標準時供給システム
- 電話回線による精密時刻比較
- 時刻同期系 (K-3型超長基線電波干渉計(VLBI)システム開発) -- (K-3型VLBI観測システム)
- 標準周波数と時刻の供給施設-2-実用標準の設定と維持 (周波数・時間標準特集号) -- (標準周波数と時刻の供給)
- Helix-Coil Transition and Micellar Structure of Poly(ethylene glycol)-block-Poly[N^5-(2-hydroxyethyl) L-glutamine] in Cyclohexanol/Water Mixed Solvents