腺癌関連糖鎖抗原に対するイディオタイプ抗体AI206の抗原認識構造に関する研究
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概要
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Monoclonal antibody (MoAb) AI206 (IgG1) is an anti-idiotypic antibody of MoAb YH206 (IgM) to adenocarcinoma-associated carbohydrate antigen and inhibits the reaction of MoAb YH206 to YH206 antigen. By Western blotting analysis, MoAb AI206 reacted with only unreduced MoAb YH206, whereas it did not react with reduced MoAb YH206. Furthermore MoAb AI206 reacted with IgM subunits (180Kd), F(ab')2 (110Kd) and Fab (50Kd) of unreduced pepsin-treated MoAb YH206. Thus MoAb AI206 recognized the structure on Fab of MoAb YH206. MoAb YH206 reacted with unreduced MoAb AI206 and also F(ab')2 (110Kd) and Fab (50Kd) of unreduced pepsin-treated MoAb AI206. It is presumed that MoAb YH206 and MoAb AI206 recognize each other in an unreduced condition but not in a reduced condition. The recognition of such a conformational idiotype on Fab must be important for this particular anti-idiotypic MoAb AI206 and MoAb YH206. Since MoAb YH206 recognized the carbohydrate on YH206 antigen as well as the peptide on MoAb AI206, the conformation on Fab of MoAb AI206 may mimic the carbohydrate structure on YH206 antigen. In fact, YH206 antibody activity was induced in syngeneic mouse serum immunized with MoAb AI206. These observations suggest that the internal image of YH206 carbohydrate antigen is preserved within the conformational idiotype on Fab of MoAb AI206. The analysis on conformational idiotype may be important for choosing an anti-idiotypic antibody which possesses the internal image of the original carbohydrate antigen.
- 1990-10-01
論文 | ランダム
- 思想の動向
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