SS-B抗原の解析とELISAによる臨床応用
スポンサーリンク
概要
- 論文の詳細を見る
SS-B antigen was purified from a saline extract of acetone powder of rabbit thymus (RTE) by a salting out method using ammonium sulfate, which was followed by affinity chromatography using Glycosyl-hardgel tagged with anti-SS-B antibody, and then the antigen fractions corresponding to anti-SS-B antibodies in patient sera were analized by Western blotting. An enzymelinked immunosorbent assay (ELISA) was developed using purified 40kDa SS-B antigen and the titers of anti-SS-B antibodies in sera were determined and compared with laboratory findings in patients with collagen diseases. The results obtained were as follows. 1) Affinity-purified SS-B antigen showed three bands, 50kDa, 40kDa and 28kDa, reactive with anti-SS-B antibody positive sera in Western blotting. The 40kDa fraction reacted only with anti-SS-B antibody, whereas the 50kDa fraction reacted with both the anti-SS-B antibody and anti-human IgG (Fc) antibody. 2) It is suggested that the 50kDa fraction has at least two antigenic epitopes: one is common to that of the 40kDa fraction reacting with anti-SS-B antibody and the other is similar to the Fc portion of human IgG. 3) The 40kDa SS-B fraction was obtained from gel slices by electrophoretical elution after SDS-polyacrylamide gel electrophoresis. Enzyme-linked immunosorbent assay using this fraction enabled us to detect IgG-, IgA-, and IgM-type anti-40kDa SS-B antibodies in sera of patients with collagen diseases. Although antibodies of all Ig classes were detected, the highest value was obtained for IgG-type. IgG-type anti-40kDa SS-B antibodies were found in 24(70.5%) of 34 patients with SjS, 13(44.8%) of 29 patients with SLE and 2(20%) of 10 patients with PSS-PM. The incidence and titer of the antibody were higher in patients of SjS than in patients other collagen diseases. In SjS and SLE, IgG-type anti-SS-B positive patients were associated with the appearance of leukopenia, lymphopenia, and hyper γ-globulinemia (p <0.05).
- 札幌医科大学の論文
- 1990-08-01
札幌医科大学 | 論文
- "すきま"の細胞生物学 -細胞間接着装置タイト結合とヒト疾患-
- 人工内耳手術における顔面神経窩の局所解剖学的検討
- 三次元MRIと高分解能CTによる蝸牛の領域分析 -人工内耳適応の術前診断への応用-
- 消化器癌転移の機序の解析と治療への応用
- 学位申請論文 ヒト気管支上皮細胞株Calu-3をモデルとしたconnexin 26による内耳機能調節のメカニズム解析