気道内Silica投与ラットにおける肺サーファクタントの変動に関する研究第2報サーファクタソト・アポ蛋白(SP36)の変動
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概要
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It is well known that pulmonary surfactant contains nonserum proteins, which are called pulmonary surfactant apoproteins. However, only a few papers have reported on the behavior of apoproteins in various pathological states. In a previous paper, we reported on the changes in phospholipid metabolism of pulmonary surfactant for rats intratracheally exposed to silica. The present paper describes the changes in apoproteins as a series of studies on the pulmonary surfactant of rats exposed to silica. Adult rats exposed intratracheally to silica (40mg/kg of body weight) were killed 28 days after silica treatment. The lung fractions were then isolated and the proteins contained in the lung fractions were analyzed. Polyclonal antibodies to rat pulmonary surfactant apoproteins were prepared, which recognized 26kD, 32kD, 38kD and 43kD proteins in control rats. In the lungs treated by silica, the protein content of extracellular surfactant fractions, i.e., P-1 and P-2, increased to 2-2.5 fold. A marked accumulation of 32kD and 38kD proteins was found in P-1 fraction which consisted mainly of cellular components such as macrophages. The P-1 fraction also contained several protein bands of small molecular weights which were not observed in control rats. These protein bands were stained by the immunoblot method using the antibodies to surfactant apoproteins. These findings indicate that pulmonary surfactant apoproteins accumulate markedly in the alveolar space of rats exposed to silica, and they are partly degradated to peptide fragments in the alveolar space. Immunohistochemical studies using the antibodies showed distinct stainings of amorphus materials and macrophages which were accumulated markedly in the alveolar space of rats exposed to silica. Alveolar type II cells which were also stained by the antibodies seemed to be somewhat atrophied probably by the pressure due to a marked accumulation of pulmonary surfactant in the alveolar space.
- 1988-04-01
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