中和モノクローナル抗体を用いたヒトロタウイルスの抗原構造の解析
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概要
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By employing 4 human rotavirus (HRV) strains, all having a different serotype specificity, we established 22 hybridomas secreting neutralizing monoclonal antibodies (N-mAbs). Reactivity patterns of these N-mAbs were examined by both neutralization and enzyme-linked immunosorbent assays using 17 rotavirus strains. Protein specificity of the N-mAbs was determined by immunoprecipitation analysis and the application of genetically reassorted HRV strains. Further, by propagating HRV KU strain in the presence of N-mAb, we prepared antigenic variants resistant to 12 different N-mAbs, and used them for epitope analysis of the HRV KU strain. The results obtained in the present study are summarized as follows: 1. Of the 22 N-mAbs obtained, 15 showed serotype-specific reactivity: 5 were specific to serotype 1, 7 to serotype 2, 2 to serotype 3 and 1 to serotype 4. The remaining 7 N-mAbs exhibited cross-reactive neutralization patterns. 2. Intraserotypic antigenic variations were found among strains belonging to each of the 4 different serotypes. In addition, a marked difference in antigenecity was shown between the serotype 2 strains and the serotype 1, 3 and 4 strains. 3. It was found that while serotype-specific antigens resided on both VP3 and VP7, crossreactive antigens were located on VP3. 4. VP7 of the serotype 1 KU strain was found to have at least 5 operationally distinct serotypespecific neutralization epitopes which constituted a single antigenic site. 5. VP3 of the KU strain was shown to have at least 4 operationally independent cross-reative neutralization epitopes.
- 1987-02-02
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