ヒトアデノウイルスF群40型 (札幌株) ゲノムのクローニング,制限酵素切断点地図の作成及びトランスホーム遺伝子の同定
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The genome of non-culturable Ad 40 (Sapporo strain) was extracted from the stool specimen of four patients with gastroenteritis and its BamHI restriction fragments were cloned. The cleavage maps were constructed for the restriction endonucleases of BamHI, EcoRI, SalI, ClaI, BclI, BstEII and PvuI. In order to examine the transforming ability of Ad40 DNA, rat 3Y1 cells were transfected with the cloned BamHI fragments of Ad40 DNA. The results obtained were as follows; 1) Cleavage maps with BamHI and EcoRI were different from those published, so that the Sapporo strain was revealed as a new genomic variant of Ad40. 2) The BamHI-F fragment showed transforming ability and the transforming gene was located in the left-end 6% of the genome. 3) The two transforming rat cell lines established showed incomplete transformation phenotypes such as fibroid morphology and the lack of growth ability in a soft-agar suspension culture. One of the cell lines, however, showed tumorigenic activity in nude mice. 4) Southern blot hybridization showed that both lines contain viral DNA sequences from 0 to 6 map units. 5) Northern hybridization detected ElA mRNA species of a nucleotide size from about 900 in the two cell lines.
- 1987-02-02
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