ブタ耳管におけるSurface Tension Lowering Substanceの検討
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概要
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Failure to maintain patency of the eustachian tube can lead to several clinical disorders of the middle ear cavity. Many researchers have reported on the eustachian tube dysfunction, but physiological function of the tube is not yet wholly understood. Pathogenesis of the eustachian tube dysfunction is still unclear, so treatment has not been established at this time. We used normal eustachian tubes of pigs in order to clarify the physiological function of the eustachian tube. Opening and closing pressures of the tube were measured before and after tubal washing. The eustachian tubal lavage was analyzed with its phospholipid and protein composition. Surface tension measurement was accomplished. The following conclusions were obtained through these experiments. 1. After washing the eustachian tube with physiological saline, opening and closing pressures were increased more than before the washing. 2. The eustachian tubal lavage was analyzed. In the surface tension measurement, eustachian tube lavage fluid appeared to have a surface activity. In phospholipid composition approximately 51% of the phospholipid was phosphatidylcholine (PC). The ratio of sphyngomyelin and phosphatidylethanolamine was 15% each. There is a similar phospholipid composition between the eustachian tube lavage and lung tissue. In the fatty acid composition of PC, palmitic acid was 36.3%. The proportion of saturated fatty acid was above half of the PC. Concerning molecular species of PC, dipalmitoyl species was 11.3%. Saturated molecular species of PC was calculated to be 16.7%.? Protein analysis was accomplished with SDS-PAGE. There were five bands in the gel which did not exist in the serum. Therefore, materials that occured in the eustachian tubal lavage fluid may be produced in the tube itself, and not derived from the serum. 3. Surface tension in phospholipid emulsion was measured. PC appeared to have the strongest surface tension activity in phospholipid classes. There was an analogous effect among the various PCs except for DPPC. 4. The measurement of opening and closing pressures was also accomplished by using phospholipid emulsions. PC was assumed to decrease the opening and closing pressures of the eustachian tube. Therefore, PC may act in maintenance of the physiological eustachian tube function. PC may play an important role in the surface tension lowering substance. 5. The eustachian tubal dysfunction causes many pathological conditions of the middle ear cavity. Phospholipid, particularly PC would be a useful agent for supplementary therapy against eustachian tubal dysfunction.
- 1986-08-01
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