Construction of Several Delection Mutants for Genes Involved in Biofilm Formation and Recovery of Heat-injured Salmonella: \Delta {agfA} and \Delta {bcsA} Mutants of Salmonella Enteritidis; \Delta {ahpC}, \Delta {ahpF}, and \Delta {katG} Mutants of S. Typ

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Salmonella is one of major food-poisoning bacteria. In food companies, controlling the bacterial growth is critically important to continue provision of good products to customers. At first, to study the attachment of the bacteria to food materials and final products, we focused on biofilm formation that is thought to be one of the reasons of the attachment. Thus, the genes related in formation of biofilm were also focused on, and \Delta {agfA} and \Delta {bcsA} mutants of Salmonella Enteritidis were constructed by using 2-step PCR and pKOBEGA helper plasmid. Secondary, we focused on heat-injured Salmonella after sublethal heat treatment as previously reported (Kobayashi et al., 2005). The heat-injured Salmonella showed expression of several specific genes to recover the bacterial functional state as well as other bacteria. To clarify the mechanism of the recovery, several gene mutants (\Delta {ahpC}, \Delta {ahpF}, and \Delta {katG} mutants of Salmonella Typhimurium, and of \Delta {rpoE}, \Delta {rpoH}, and \Delta {rpoS} mutants of S. Enteritidis and S. Typhimurium) were constructed.
2009-10-29