dsRNA Binding Activity of Silworm Larval Hemolymph is Mediated by Lipophorin Complex
スポンサーリンク
概要
- 論文の詳細を見る
RNAi induction by the injection of long dsRNA into hemocoel has been tried to archive efficient gene knockdown in silkworm larvae or pupae, and severas reports successfully applying RNAi to silkworm have been brought out. However, effect of RNAi in silkworm is often insufficient and improvement of efficiency is desirable. On the other hand, the metabolic turnover or organ uptake of dsRNA injected into insects has not been sufficiently clarified so far. In this study, we report that silkworm larval hemolymph has dsRNA binding activity. We also show that the molecular entity of dsRNA binding activity of hemolymph is lipophorin, a major lipoprotein complex in insects. dsRNA binding activity of silkworm larval hemolymph was observed by electrophoretic mobility shift assay. dsRNA binding activity-associated protein was purified by affinity chromatography with immobilized dsRNA as a ligand. Amino acid sequence of the purified protein was determined by Edman degradation and the protein was revealed to be a silkworm homolog of Apolipophorin I, an unexchangeable apolipoprotein subunit of lipophorin. Lipophorin fraction prepared from hemolymph by density gradient ultracentrifugation also exhibited the similar dsRNA binding activity. We determined full-length sequence of Apolipophorin II/I precursor homolog of silkworm, whose structure was highly conserved among insects. Although physiological role of dsRNA binding activity is still unknown, the present study indicates potential effect by injection of dsRNA into silkworm individuals.
- 2009-10-29
論文 | ランダム
- 自律神経機能評価のためのウェーブレット変換による心拍変動の時間周波数解析
- 歩行解析における股関節中殿筋の質的評価の試み : ウェーブレット変換による動的周波数解析
- 動的バランス訓練システムの開発 : インターネットを使用して
- Synthetic Studies on Indoles and Related Compounds. XXV. The Friedel-Crafts Acylation of Ethyl 1H-Indole-2-carboxylate. (2)
- 308. インターネットを利用した動的バランス訓練システムの開発