Deposition onto Glass Surface of Two-dimensional Crystal Film of Streptavidin

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原著論文1. Introduction2. Experimental2.1 Materials2.2 Preparation and transfer of 2D crystal film of streptavidin2.3 Thermal treatment of the glass coverslip2.4 Atomic force microscopy3. Results and Discussion3.1 Surface roughness of glass coverslip, fused silica plate and silicon wafer3.2 Deposition of PBLH/SA 2D crystal film onto thermally treated glass coverslip3.3 Protein G/IgG assembly fabricatedThe two-dimensional (2D) crystal film of streptavidin (SA) prepared at the air/water interface had been transferred onto a hydrophobic surface of silicon wafer and studied by atomic force microscopy1). In the present study the deposition of this film onto glass coverslip has been examined. Annealing the glass coverslip at 525 ºC rendered the surface smoother and stabilized adhesion of the transferred film. The use of glass as substrate for protein immobilization has the advantage that an optical microscope can be used in transmission mode, and the fluorescence quenching which generally occurs for dyes locating near metal or semiconductor surfaces can be avoided2）. Therefore, the 2D crystal film of SA deposited on glass would find wider applications than on silicon as the protein binding base using biotinylated proteins.
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