アルカリ性ピクリン酸法及び高速液体クロマトグラフィによる尿中クレアチニンの定量
スポンサーリンク
概要
- 論文の詳細を見る
Urinary creatinine has been widely analyzed in research fields concerning body composition and muscular activities since the amount of creatinine excretion in urine is nearly proportional to the muscular mass in humans, and the amount in an individual is nearly constant. And also research studies pointed out the decrease in urinary creatinine after acute exercise. Effects of exercise upon urinary creatinine have been, furthermore, reported. However, there are research studies indicating that Borsook method shows slightly lower values than Folin method and that the liquid chromatographic procedure gives results that are lower than those obtained by an alkaline picrate method(APM) and the procedure is more precise. The purpose of this study is, therefore, to clarify the preciseness of APM in a case of determining urinary creatinine. Thirty one healthy college male students volunteered to be subjects in this study. Urinary creatinine values by APM was compared with those by a high performance liquid chromatography(HPLC). A Folin-Wu method was used for APM. This study found that urinary creatinine values by HPLC is significantly lower than those by APM as Patel and George indicated although there was a significant correlation between the values by HPLC and APM. And also it was found that a significant regression formula, y=1.0168x-118.099(where x=urinary creatinine concentration by APM),should be used to obtain more precise creatinine concentration in urine which is y values, urinary creatinine concentration by HPLC, if HPLC is not available.
論文 | ランダム
- モデリングに及ぼす依存度と方略使用パタンの効果
- 観察前における課題構造の理解水準とモデリング効果との関係
- 観察前における課題構造の理解水準とモデリング効果との関係
- 603 子供は教示と観察からどれほど学習できるのか(教授・学習1,教授・学習)
- Production and characterization of a large population of cDNA-overexpressing transgenic rice plants using Gateway-based full-length cDNA expression libraries